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degenerate primers

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  • degenerate primer — A single stranded synthetic oligonucleotide designed to hybridize to DNA encoding a particular protein sequence. As the mapping of codons to amino acids is many to one, the oligonucleotide must be made as a mixture with several different bases at …   Dictionary of molecular biology

  • Primer (molecular biology) — A primer is a strand of nucleic acid that serves as a starting point for DNA synthesis. They are required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of… …   Wikipedia

  • Variants of PCR — This page assumes familiarity with the terms and components used in the Polymerase Chain Reaction (PCR) process. The versatility of PCR has led to a large number of variants: Contents 1 Basic modifications 2 Pretreatments and extensions 3 Other… …   Wikipedia

  • Primer Premier — Infobox Software name = Primer Premier caption = developer = Premier Biosoft latest release version = 5.0 latest release date = 6 May 2008 latest preview version = latest preview date = operating system = Windows platform = genre = Bioinformatics …   Wikipedia

  • Polymerase chain reaction optimization — The polymerase chain reaction (PCR) is a commonly used molecular biology tool for amplifying DNA, and various techniques for PCR optimization have been developed by molecular biologists to improve PCR performance and minimize failure. Contents 1… …   Wikipedia

  • OLIGO Primer Analysis Software — Developer(s) Molecular Biology Insights, Inc. Stable release 7.54 / March 23, 2011 Operating system Windows, Macintosh Platform Mac, PC …   Wikipedia

  • Nucleic acid analogues — Not to be confused with degenerate bases. For phosphoramidite synthesis of nucleic acids, see Oligonucleotide synthesis. RNA with its nucleobases to the left and DNA to the right. Nucleic acid analogues are compounds structurally similar (analog) …   Wikipedia

  • inosine — The fifth base of nucleic acids. Important because it fails to form specific pair bonds with the other bases. In transfer RNAs, this property is used in the anticodon to allow matching of a single tRNA to several codons. PCR performed with… …   Dictionary of molecular biology

  • Transglutaminase — La transglutaminase est une enzyme (EC 2.3.2.13) qui catalyse la formation de liaisons covalentes entre des groupements amines libres (ex. : lysines) et le groupement gamma carboxamide des glutamines. Les liaisons formées par la… …   Wikipédia en Français

  • Transglutaminase — Transglutaminases are a family of enzymes (EC 2.3.2.13) that catalyze the formation of a covalent bond between a free amine group (e.g., protein or peptide bound lysine) and the gamma carboxamid group of protein or peptide bound glutamine. Bonds… …   Wikipedia

  • TGM3 — For Tetris clone, see . Transglutaminase 3 (E polypeptide, protein glutamine gamma glutamyltransferase), also known as TGM3, is a human gene.cite web | title = Entrez Gene: TGM3 transglutaminase 3 (E polypeptide, protein glutamine gamma… …   Wikipedia

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